Identification of pathogenic bacteria in the storage period of pomegranate in Liangshan state

Abstract:【Objective】In order to systematically study the disease of pomegranate storage in Huili Countyof Liangshan Yi Autonomous Prefecture, and clarity the pathogen species.【Methods】The isolates werecollected and purified according to the Koch’s law, separation, inoculation and re-purification. The patho⁃gen was identified according to its morphological characteristics and rDNA-ITS sequence analysis.【Results】According to the symptoms of pomegranate storage in Liangshan Prefecture, it was divided intothree types: dry rot and soft rot, and soft rot was divided into three groups. Four dominant strains were isolated from four disease types, B, C, D. The morphological characteristics and the rDNA-ITS sequenceanalysis showed that there were four major pathogens in the pomegranate storage period in Liangshan Prefecture, namely, Coniella granati, Penicillium glabrum, Aspergillus niger, and Botrytis cinerea.【Conclusion】Combining morphological characteristics and rDNA-ITS sequence analysis, the pathogens of dry rotfrom Liangshan state were identified as Coniella granati, and the pathogens of soft rot were identified asPenicillium glabrum, Aspergillus niger, and Botrytis cinerea.