Abstract: 【Objective】Due to factors such as harvest maturity and storage conditions, the core of Yuluxiang pear is prone to browning during long-term storage, becoming the main factor affecting its quality after storage. Ice temperature storage is a storage method that stores fruits between 0 ℃ and their freezing point, which can effectively delay fruit metabolism, reduce occurrence of fruit browning, maintain the quality and color of Yuluxiang pear. Metabolomics can use detection and analysis of types, quantities, and changes of metabolites to find relative relationship between metabolites and physiological and pathological changes during a specific physiological period and under specific conditions. Therefore, untargeted metabolomics with metabolic profiling analysis can be used to analyze the changes in metabolites during browning process of Yuluxiang pear, and further explore the impact of ice temperature technology on it, to provide theoretical reference for improving the mechanism and control of pear browning.【Methods】Using near-infrared fruit non-destructive detector, Yuluxiang pear with soluble solid content of 11.5%-12.5% were selected as the test material. After pre-cooling, they were packaged in high permeability CO2 polythene fresh keeping bag and stored at -1.0±0.5 ℃ and 0.0±0.5 ℃, respectively. Regular sampling was conducted to measure the core browning index, total phenols, total flavonoids and polyphenol oxidase enzyme activity at every 60 days of cold storage. Each sample was represented by three biological replicates. Ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was used to perform untargeted metabolomics analysis on the core of Yuluxiang pear after 240 days stored at -1 ℃ and 0 ℃. The metabolomics samples were Y-C (0 day post harvest), Y-S-C (stored at 0.0 ± 0.5 ℃ for 240 days), Y-I-C (stored at -1.0 ± 0.5 ℃ for 240 days). By using multivariate statistics such as principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), the kyoto encyclopedia of genes and genomes (KEGG) database, differential metabolites and metabolic pathways were screened and analyzed. Combining with the determination of physiological indexes, the effect of ice temperature on pear core browning was analyzed.【Results】The results showed that the core browning occured 120 days after harvest of cold storage at 0 ℃ and the browning symptoms only occurred in the pear corn during stoage period. With the increase of polyphenol oxidase activity, total phenol content in the core of Yuluxiang pear at 0 ℃ gradually decreased, and total flavonoid content also showed a downward trend. Ice temperature storage at -1 ℃ delayed the decrease in total phenolic and total flavonoid content, maintained a certain level of antioxidant capacity, inhibited the increase in polyphenol oxidase activity, lightened core browning. Metabolomics analysis revealed that a total of 331 metabolites with different relative contents were obtained in Yuluxiang pear core tissue before and after storage, which were mainly classified into flavonoids(72), phenylpropanoids(56), terpenes(44), sugars and polyols(40), alkaloids(37), organic acids(15), lipids(13), amino acids and their derivatives(10), tannins(4), quinones(4), nucleotides(2) and other unclassified compounds(37). Among them, the higher relative content of metabolites were organic acids, sugars and polyols and alkaloids. In the Y-S-C vs Y-C samples, 31 differentially expressed metabolites were identified based on a variable importance in the projection(VIP) > 1.0 and t-test<0.05, which 4 metabolic compounds were up-regulated and 27 metabolic compounds were down-regulated. Compared with Y-C treatment, phenylpropanoid substances such as protocatechuic acid, catechol, dendrophenol and ferulic acid were down-regulated after cold storage at 0 ℃, and organic acids such as trifluoroacetic acid, 2-isopropylmalic acid, and methylsuccinic acid were down-regulated, sugars and polyols, and flavonoids were all down-regulated as well. These differential metabolites were mapped to 11 KEGG pathways, among these pathways, valine, leucine and isoleucine biosynthesis, pyruvate metabolism, and fructose and mannose pathways were significantly enriched. 50 species differentially expressed metabolites were identified in Y-I-C vs Y-S-C samples, which the numbers of up-regulated and down-regulated metabolites were 26 and 24 respectively. There are 11 phenylpropanoids, among which catechol, arbutin, protocatechuicaldehyde, quinic acid, and ferulic acid were up-regulated in expression levels when stored at -1 ℃ compared to 0 ℃. There are 7 organic acid substances, among which malic acid, 2-isopropylmalic acid, trifluoroacetic acid, and citric acid were up-regulated in expression levels when stored at -1 ℃ compared to 0 ℃. Other types include sugars and polyols(8), terpenes(6), flavonoids(5), lipids(3), amino acid(2), alkaloids(2), quinone(1) and others(5). The KEGG enrichment pathway enriched 50 significantly different metabolites in 25 metabolic pathways, among which the more concentrated pathways included metabolic pathways, amino acid metabolism, biosynthesis of other secondary metabolites, carbohydrate metabolism, as well as lipid metabolism, metabolism of cofactors and vitamins, environmental information processing, metabolism of terpenoids and polyketides, membrane transport and other pathways.【Conclusion】 Ice temperature storage(-1℃) delayed the increase of polyphenol oxidase activity and the oxidation of total phenols and flavonoids, inhibiting core browning of Yuluxiang pear. 50 species differentially expressed metabolites were screened out in the pear core, and 26 substances were mainly up-regulated, including phenylpropanoids, flavonoids, organic acids, etc. Through KEGG metabolic pathway enrichment analysis, it was found that they significantly contribute to metabolic pathways, amino acid metabolism, and biosynthesis of other secondary metabolites. In addition, TCA cycle, glycolysis/gluconeogenesis, and fatty acid degradation were also involved. Ice temperature treatment increased the antioxidant capacity of fruits by up-regulating substances such as catechol, arbutin, and citric acid and synergistically affected the oxidative metabolism of the fruit core through related pathways.
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