Progress in the study on in vitro regeneration and genetic transformation in pears (Pyrus spp.)

Abstract：The current national and international progress is summarized in the regeneration and genetic transformation of pear explants.The effect of growth hormone ratios, genotypes, basic culture media and AgNO3 on the regeneration efficiency and factors affecting the transformation rate in the Agrobacterium-mediated transformation were analyzed.It is believed that the genotype is the most limiting factor for the regeneration rate as well as the transformation efficiency of pear explants. As the methods used for different pear cultivars are various, therefore, it is the first step to establish a genetic transformation system suitable for one's own experiment in the study on molecular biology of pears.Through summarizing and analyzing the research method and results obtained by some previous researchers in the field, we have attempted to find common obstacles and important solutions in the genetic transformation of pears, so as to provide the new researchers with valuable ideas for the future research on molecular breeding of pears.