Cloning and expression analysis of Ran gene in different tissues and under low temperature stress in Sanming yesheng jiao(Musa itinerans)

【Objective】The objective of the study is to elucidate the mechanism of Ran gene involved in response to low temperature stress.【Method】The c DNA sequence of Ran gene in Yesheng jiao from Sanming was isolated by reverse transcription polymerase chain reaction(RT-PCR) with rapid amplification of cDNA ends(RACE). The expression of this gene in response to low temperature stress and under salicylic acid treatment was determined by real-time quantitative PCR(q RT-PCR).【Result】6 Ran gene cDNA sequences that containing complete open reading frames were isolated from leaves of Sanming yesheng jiao(Musa itinerans Cheesman). All of them encode proteins that show high homology with Ran proteins from other plant species and contain characteristic domains of the Ran proteins including GTP Hydrolysis domain,Ran GAP-binding domain and acidic tail. Results of q PCR analysis indicated that Ragenes were expressed in root,leaf,peduncle,bract,flower,peel and pulp in Sanming yesheng jiao. The lowest m RNA expression was found in root,and the higher expression in leaf,flower and pulp. In addition,the expression levels of Ran genes were affected significantly by low temperature stress and under salicylic acid treatment in wild musa from Sanming.【Conclusion】Ran genes were involved in cell division and responses to low temperature stress and salicylic acid signal transduction in Sanming yesheng jiao.