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Home-Journal Online-2015 No.3

The SSR fingerprinting of grapevine cultivars newly-developed in China

Online:2018/5/30 16:49:36 Browsing times:
Author: YIN Ling ZHANG Chen XIANG Jiang ZHANG Yali AN Yunhe XU Haiying ZHAO Shengji-an GUO Xiuwu LU Jiang
Keywords: Grapevine; SSR marker; DNA fingerprint;
DOI: 10.13925/j.cnki.gsxb.20140380
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PDF Abstract

【Objective】To fingerprint and define the genetic relationship of grapevine cultivars newly-de-veloped in China.【Methods】We genotyped 24 new cultivars with six pairs of international fluorescence-labeled SSR primers by capillary electrophoresis.【Results】The results indicated that not only each culti-vars tested but also their ploidy levels can be distinguished clearly with these six universal primers basedon these SSR fingerprinting results. At the same time,these markers can also be used to identify the rela-tionship between different varieties,distinguish the grape cultivars from different parents,populationsand subgenus.【Conclusion】The establishment of this SSR fingerprint can perfect the grape varieties data-base in our country,and can also provide a good reference for cultivar identification,plant varieties pro-tection and genetic relationship analysis.