Cloning and sequence analysis of MiLCYB2 gene promoter from mango(Mangifera indica L.)

【Objective】To study the expression and regulation rule of Mi LCYB2 in mango.【Methods】A991 bp fragment of Mi LCYB2 promoter was cloned by chromosome walking method.【Results】Promoter se-quence analyzed by Plant CARE and PLACE showed that this promoter fragment had TATA-BOX, CAAT-BOX and some other regulatory elements, such as a variety of plant hormone cis-acting element involvedin the abscisic acid responsiveness, the gibberellic acid responsiveness, the auxin responsiveness, the sali-cylic acid responsiveness and the Me JA responsiveness; and also cis-acting element was involved in circa-dian control, MYBHv1 binding site and light responsive element. It was speculated that the expression of Mi LCYB2 was regulated by a variety of plant hormone, light, circadian, MYBHv1 transcription factors etal.【Conclusion】Isolation and sequence analysis of Mi LCYB2 promoter will help further explore the regu-lation of carotenoid biosynthesis pathway in mango.