- Author: FENG Hu, ZHANG Ying, FAN Xiucai, JIANG Jianfu, SUN Haisheng, LIU Chonghuai
- Keywords: Chinese wild grape Vitis davidii 0943; Transcription factor; Vd WRKY49; White rot fungi;
- DOI: 10.13925/j.cnki.gsxb.20150050
- Received date:
- Accepted date:
- Online date:
PDF （） Abstract（）
Abstract: 【Objective】To analyze the relationship between VdWRKY49 sequence feature,gene function and resistance to grape white-rot,and reveal the molecular regulatory mechanisms of the resistant germplasm. 【Methods】According to the homologous sequences Vv WRKY49,primers were designed and used to clone Vd WRY49 of Vitis davidii 0943. The resistant founction of Vd WRY49 to grape white-rot was confirmed by bioinformatics analysis and real-time quantitative PCR. The relative expression of WRKY49 of was detected by real-time PCR in V. davidii 0943 and‘Pinot Noir'after white rot fungi inoculation and salicylic acid treatment.【Results】Some resistance gene features were found at the transcribed region and promoter region of VdWRKY49 from Chinese wild grape V. davidii 0943. In addition,the nucleic acid and animo acid sequence of WRKY49 have differences between V. davidii 0943 and‘Pinot Noir',which may cause the changes of protein structure and function. The expression of VdWRKY49 is higher than Vv WRKY49 after treatment with white rot fungi and salicylic acid.【Conclusion】The expression pattern of VdWRKY49 are distinguished from Vv WRKY49 obviously,indicating that VdWRKY49 plays an importantbiological function in grape disease resistance.