Identification and comparison of differential metabolites from the skin of mature fruit in‘Huanghua'pear and its bud mutant‘Lühuanghua'pear(Pyrus pyrifolia Nakai)

【Objective】The aim of this study was to discover differential metabolites from the skin of ma-ture fruit in‘Huanghua'and its bud mutant‘Lühuanghua'(Pyrus pyrifolia Nakai).【Methods】The me-tabolites in skin were measured by gas chromatography-mass spectrometer(GC-MS)and liquid chroma-tography-mass spectrometer(LC-MS). The data were analyzed using principal component analysis andpartial least squares-discriminate analysis(PLS-DA).【Results】Differences in pear skin color resultedfrom the significant difference of metabolite. Eighteen differential metabolites were found by GC- MS,mainly including organic acids(Oxalic acid,Malic acid and Citric acid),amino acids(Alanine and L-Asparagine)and sugar(D-Fructose,D-Glucose and Sucrose). Forty-three differential metabolites wereobtained by LC-MS,primarily including fatty acids(cis-15-tetracosenoic acid,5,9-tetracosadienoic ac-id,linoleic acid,linolenic acid,stearic acid,docosanoic acid and tetracosanoic acid),esters(dihexyl1,2-benzenedicarboxylate and 2-Monolinoleoylglycerol),triterpenoids(ursolic acid and squalene),phenolics(5-O-trans-p-coumaroyl quinic acid,vanillic acid,chlorogenic acid and arbutin,),flavo-noids,phenylpropanoids(coniferyl aldehyde and cinnamic acid),sterols(Stigmasterol)and vitamin E(δ-tocotrienol and α-tocotrienol).【Conclusion】The difference of skin color between‘Huanghua'andits bud mutatant‘Lühuanghua'might be caused by the synergistic reaction of these differential metabo-lites.