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Home-Journal Online-2015 No.6

Genetic analysis of local Citrus germplasm resources in Guangxi by ISSR markers

Online:2018/5/16 10:07:30 Browsing times:
Author: LIU Binghao DENG Chongling CHEN Chuanwu DENG Guangzhou DING Ping NIU Ying TANG Yan FU Huimin
Keywords: Citrus resources; Guangxi; Local; ISSR; Genetic diversity;
DOI: 10.13925/j.cnki.gsxb.20140407
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PDF Abstract

【Objective】To elucidate the genetic relationship and genetic diversity of Guangxi local Citrus resources.【Methods】In this study,ISSR molecular marker techniques was used to detect the genetic di-versity and relationship of 10 local Citrus and 18 wild Citrus and resources were using.【Results】A total of67 bands were generated from 12 ISSR primers,of which 92.54% was polymorphic. As analyzed by NT-SYS-pc,all the 28 resources can be divided into seven groups,and parts of Guangxi resources into sixgroups. Eight Guposhan wild Citruses are clustered into the same group,and thus can be divided into twosubgroups.【Conclusion】Guangxi local Citrus can be distinguished by ISSR markers,and the genetic di-versity of citrus in Guangxi is rich.