Abstract: 【Objective】To obtain the complete genomic sequences of grapevine pnot gris virus (GPGV) isolate from Inner Mongolia, and to perform analyses on the viral population concerning sequence identity, phylogenetic relationships, genetic recombination, and genetic diversity. 【Methods】The complete genomic sequences of the GPGV Inner Mongolia isolate were cloned using RT-PCR and rapid amplification of cDNA ends (RACE) techniques, with GPGV positive samples serving as the experimental material. Subsequent genomic sequence analyses were conducted using molecular biology software. 【Results】Two complete genomic sequences of the GPGV Inner Mongolia isolates (20IM-ViVi1 and 20IM-ViVi2) were cloned, with both having a full length of 7250 nucleotides and encoding three open reading frames (ORFs). Sequence identity analysis revealed that the genomic sequences of 20IM-ViVi1 and 20IM-ViVi2 exhibited 96.4% nucleotide identity, while their identities with other isolates' complete genomic sequences ranged from 79.7% to 96.8% and from 79.5 to 97.7%, respectively. Phylogenetic analysis indicated that all GPGV complete genomic sequences could be classified into four clades, with the two isolates from this study, 20IM-ViVi1 and 20IM-ViVi2, grouped in the first clade and showed the closest genetic relationship to Vitis vinifera cultivar Summer Black isolate SRR2845691-GPGV. Genetic diversity analysis demonstrated that GPGV possesses a high level of genetic diversity, with the Asian isolates displaying the highest level. 【Conclusion】This study represents the first time the complete genomic sequences of GPGV isolates from Inner Mongolia have been obtained, and it elucidates the evolutionary relationships between the two GPGV Inner Mongolia isolates and known viruses, providing a foundational theory for the classification of GPGV strains and genetic evolutionary studies in China.
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