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Anatomical Observation of the Differences Between the Fruit Texture of Z. jujuba cv. Huizao and Z. jujuba cv. dongzao and Related Enzyme Activities

Date:2024/7/26 15:03:28 Visit:

Abstract: ObjectiveIn this study, the microstructure of fruit pulp cells was observed in this study of two jujube(Zizyphus Jujuba Mill.) varieties with obvious differences in fruit texture and texture, and the water content, hardness and activity of cell wall-related metabolic enzymes were measured, and correlation analysis was carried out to explore the key factors affecting the formation of the two textures.MethodsThe moisture content, hardness, pulp cell morphology, cell wall components and metabolic enzyme activities of dried jujube Z. jujuba cv. huizao and fresh jujube Z. jujuba cv. dongzao were used as test materials after 31 days (S1), 63 days (S2), 78 days (S3), 98 days (S4) and 108 days (S5) after flowering. Results(1) As the fruit develops, the water content of both Z. jujuba cv. huizao and Z. jujuba cv. dongzao shows a trend of first increasing and then decreasing, hardness shows a trend of decreasing, and Z. jujuba cv. huizao hardness is persistently higher than that of Z. jujuba cv. dongzao. (2) The two varieties of its pulp cell changes are presented first rapid increase and then slowly increase trend, but Z. jujuba cv. dongzao pulp thin-walled cell area is significantly larger than the Z. jujuba cv. huizao pulp thin-walled cell area. (3) Fruit pulp cell ultrastructural observation showed that at the S5 period, the two varieties of fruit pulp cell wall, the gel layer has been obviously degraded, but, the degradation of gel layer and inclusions in Z. jujuba cv. dongzao is slower compared to Z. jujuba cv. huizao. (4) The total pectin and cellulose content of the two varieties as a whole showed a trend of gradual decrease, and the Z. jujuba cv. dongzao was always lower than the Z. jujuba cv. huizao. (5) Fruit hardness was significantly and positively correlated with total pectin and cellulose content, and highly significantly and negatively correlated with β-Galα-Af and PL enzyme activities, and the correlation between PG enzyme and Z. jujuba cv. dongzao fruit hardness and water content was highly significant, but the correlation with Z. jujuba cv. huizao was not significant.ConclusionThe difference in texture quality between Z. jujuba cv. huizao and Z. jujuba cv. dongzao is related to the differences in total pectin and cellulose content of cell wall fractions and the microstructure of pulp cells, and is also related to the cell wall metabolism-related enzymes β-Galα-Af and PL enzymes; moreover, the PG enzyme plays a key role in the Z. jujuba cv. dongzao texture changes also play a key role.



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