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Home-Journal Online-2018 No.s1

The identification and the Pathogenicity of analysis for the pathogen of pear Valsa canker

Online:2019/11/22 14:55:26 Browsing times:
Author: DONG Ran, WEI Shuwei, RAN Kun, WANG Hongwei, DONG Xiaochang, WANG Shaomin
Keywords: Pear; Shandong; Valsa mali; Isolation; Pathogenicity;
DOI: 10.13925/j.cnki.gsxb.2018.S.22
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Abstract:【Objective】In order to detect the resistance of pear germplasms to canker, we obtained fungal pathogens from pear trees.【Methods】Isolates from disease-affected branches of pear were purified culture and obtaining pear rot disease strain named (No.Va1) .Identification of the pathogenic fungus was carried out according to the characteristics of fungal morphology, pathogenicity and by rDNA-ITS sequence analysis.【Results】The strain was identified as Valsa mali based on the colony and conidiophores morphological observation and the rDNA-ITS sequence homology.Eight pear germplasm resources were selected for pathogenicity detection.【Conclusion】The results showed that the isolate was the pathogen of pear rot.It has certain pathogenicity of the Va1 isolate on eight varieties of pear.