Comparison and quality analysis of total RNA extraction methods from different tissues of Pyrus betulaefolia Bunge

Abstract：【Objective】Aiming at the abundant amounts in amylose, hydroxybenzene and other secondary metabolities in the tissues of Pyrus betulaefolia Bunge, the study was undertaken to screen out the method in order to obtain the high-quality RNA.【Methods】The different tissues (root, stem, leaf, flower and fruit) were used as the materials, and the total RNA was extracted by Trizol method, the improved CTAB method and RNAprep Pure Plant Kit method.The concentration and purity of the extracted RNA were analyzed by Agarose Gel Electrophoresis and NanoDrop 2000.【Results】The total RNA can be extracted by the 3 methods, and the bands of the total RNA extracted by RNAprep Pure Plant Kit method were clear.The purities were 2.07, 2.02, 2.01, 2.01 and 2.02, respectively, and the concentrations were186.2 ng· μ L-1, 174.5 ng· μ L-1, 163.6 ng· μ L-1, 167.3 ng· μ L-1 and 121.1 ng· μ L-1 respectively.Additionally, the total RNA extracted with RNAprep Pure Plant Kit method could be used for reverse transcription, and the amplification bands of PbUBI gene were specific.【Conclusion】The RNAprep Pure Plant Kit method was suitable for different tissues of Pyrus betulaefolia Bunge, which laid the foundation for the further molecular biology research.