- Author: ZHANG Xiaohui HUAGN Chunhui ZHONG Min CHEN Chujia LANG Binbin QU Xueyan XU Xiaobiao
- Keywords: Yellow flesh kiwifruit; Carotenoids; RNA-seq; Differences in gene; Relative expression levels;
- DOI: 10.13925/j.cnki.gsxb.20140485
- Received date:
- Accepted date:
- Online date:
PDF （） Abstract（）
【Objective】To explore the role of related difference genes in the carotenoid biosynthesis in yel-low-fleshed kiwifruit.【Methods】Based on RNA-seq,a yellow flesh cultivar‘Jinfeng'(Actinidia chinensis)was used to explore the carotenoid synthesis related gene expression through q PCR technique.【Re-sults】Four genes(Unigene23885,CL10798,Unigene11266 and CL1511)had much stronger expres-sion,and correlation analysis showed a significantly positive correlation existed between the carotenoidcontents and four gene(Unigene11266,Unigene23885,Unigene2673)(P< 0.05) and Unigene23823,Unigene12775(P<0.01) expressions. The cluster analysis showed that Unigene23885 and Unigene11266,gene CL1511 and CL10798 were clustered together and belong to the same category,but differ-ent from other genes as they had the same expression pattern in which it was significantly different fromother genes,gene CL10467 was nearest with them.【Conclusion】Based on all the above results,Unige-ne11266 and Unigene23885 might be the key genes during the carotenoid synthesis in yellow flesh kiwi-fruit,and the genes CL10467,Unigene12775,CL10798,CL1511 and Unigene2673 had a closer rela-tionship carotenoid synthesis,compared with other genes.