Study on extraction and antioxidant activity of anthocyanins from blueberry pomace

【Objective】The objective of the study is to develop the basis for comprehensive utilization ofanthocyanin from blueberry pomace.【Methods】The influences of ethanol concentration,solvent-materialratio,time,p H and temperature on the anthocyanins content were investigated in single-factor experi-ments. The process parameters were optimized by response surface analysis method. In addition,the anti-oxidant activity of anthocyanin was evaluated based on total antioxidant power,DPPH and OH radical-scavenging assay.【Results】The results showed that ethanol concentration,solvent-material ratio,time,p H and temperature had great impact on content of the anthocyanins,the optimum extraction conditionwere ethanol concentration 60%,25∶1 of solvent-material ratio,1.5 h of time,p H 2.0 and 35 ℃ of tem-perature by response surface analysis method. The content of the anthocyanin was 16.197 mg·g- 1by themethod. The anthocyanin from blueberry pomace displayed dose-dependent reducing power and abilitiesto scavenge DPPH and hydroxyl free radicals,and IC50 values of DPPH·and OH· were 241.88 mg·L- 1and 1 238.15 mg·L- 1.【Conclusion】The extraction condition of anthocyanin from blueberry pomace wasoptimized and anthocyanins from blueberry pomace showed good antioxidant activities.